袁必锋

博士 , 教授
研究方向: 色谱-质谱分析 / 生物分析化学
联系电话: 化西109B
Email: bfyuan@whu.edu.cn

 

 

教育与研究经历

1997. 9 - 2001. 6    武汉大学 生命科学学院 生物化学专业   学士

2001. 9 - 2006. 6    武汉大学 生命科学学院 生物物理专业   博士

2006. 11 - 2007. 6  新加坡国立大学 生物系                         博士后

2007. 7 - 2011. 1    美国加州大学河滨分校 化学系              博士后

2011. 2  至今          武汉大学 化学与分子科学学院               教授/博导

 

研究领域与兴趣

1. 核酸表观遗传修饰(Epigenetic Modifications)

核酸表观遗传修饰不仅在基因调控等方面有重要作用,而且在癌症等许多疾病的发生中也扮演着重要角色。本课题组通过建立质谱分析等研究方法,对核酸表观遗传修饰的生理功能进行研究。

2. 代谢组分析(Metabolomics

发展基于质谱技术的代谢组学研究方法,结合相应的生物学手段,探索代谢物在疾病发生、发展过程中的生理功能。 

 

本课题组致力于化学和生物学交叉领域的研究工作,欢迎具有化学或者生物学背景的学生、博士后加入。

 

项目

1. 自然科学基金面上项目(21672166)

2. 自然科学基金优秀青年基金(21522507)

3. 自然科学基金青年基金(21205091)

4. 自然科学基金海外及港澳学者联合基金(21228501)

5. 国家重点基础研究发展计划(973 计划)子课题(2012CB720601)

 

课程

本科生课程:《化学生物学》

研究生课程:《生物分析化学》

 

代表性论文

  1. Huang, W., Lan, M.D., Qi, C.B., Zheng, S.J., Wei, S.Z., Yuan, B.F.* and Feng, Y.Q. (2016). Formation and determination of the oxidation products of 5-methylcytosine in RNA. Chem Sci. 7, 5495-5502.

  2. Zhang, H.Y., Xiong, J., Qi, B.L., Feng, Y.Q.* and Yuan, B.F.* (2016) The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals. Chem Commun (Camb), 52, 737-740

  3. Huang, W., Qi, C.B., Lv, S.W., Xie, M., Feng, Y.Q.*, Huang, W.H.* and Yuan, B.F.* (2016) Determination of DNA and RNA Methylation in Circulating Tumor Cells by Mass Spectrometry. Anal Chem. 88, 1378-1384.

  4. Tang, Y., Zheng, S.J., Qi, C.B., Feng, Y.Q.* and Yuan, B.F.* (2015) Sensitive and simultaneous determination of 5-methylcytosine and its oxidation products in genomic DNA by chemical derivatization coupled with liquid chromatography-tandem mass spectrometry analysis. Anal Chem, 87, 3445-3452.

  5. Chu, J.M., Qi, C.B., Huang, Y.Q., Jiang, H.P., Hao, Y.H., Yuan, B.F.* and Feng, Y.Q.* (2015) Metal Oxide-Based Selective Enrichment Combined with Stable Isotope Labeling-Mass Spectrometry Analysis for Profiling of Ribose Conjugates. Anal Chem, 87, 7364-7372.

  6. Shen, F., Huang, W., Huang, J.T., Xiong, J., Yang, Y., Wu, K., Jia, G.F., Chen, J., Feng, Y.Q.*, Yuan, B.F.*, Liu, S.M.* (2015) Decreased N(6)-Methyladenosine in Peripheral Blood RNA From Diabetic Patients Is Associated With FTO Expression Rather Than ALKBH5. J Clin Endocrinol Metab, 100, E148-154.

  7. Yuan, B.F.* and Feng, Y.Q. (2014) Recent advances in the analysis of 5-methylcytosine and its oxidation products. TrAC-Trend Anal Chem, 54, 24-35.

  8. Xing, X.W., Tang, F., Wu, J., Chu, J.M., Feng, Y.Q., Zhou, X.* and Yuan, B.F.* (2014) Sensitive detection of DNA methyltransferase activity based on exonuclease-mediated target recycling. Anal Chem, 86, 11269-11274.

  9. Tang, Y., Xiong, J., Jiang, H.P., Zheng, S.J., Feng, Y.Q.* and Yuan, B.F.* (2014) Determination of oxidation products of 5-methylcytosine in plants by chemical derivatization coupled with liquid chromatography/tandem mass spectrometry analysis. Anal Chem, 86, 7764-7772.

  10. Qi, B.L., Liu, P., Wang, Q.Y., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Derivatization for liquid chromatography-mass spectrometry. TrAC-Trend Anal Chem, 59, 121-132.

  11. Liu, P., Huang, Y.Q., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Profiling of thiol-containing compounds by stable isotope labeling double precursor ion scan mass spectrometry. Anal Chem, 86, 9765-9773.

  12. Wang, S.T., Huang, W., Lu, W., Yuan, B.F.* and Feng, Y.Q.* (2013) TiO2-Based Solid Phase Extraction Strategy for Highly Effective Elimination of Normal Ribonucleosides before Detection of 2''-Deoxynucleosides/Low-Abundance 2''-O-Modified Ribonucleosides. Anal Chem, 85, 10512-10518.

  13. Tang, Y., Chu, J.M., Huang, W., Xiong, J., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Hydrophilic material for the selective enrichment of 5-hydroxymethylcytosine and its liquid chromatography-tandem mass spectrometry detection. Anal Chem, 85, 6129-6135.

  14. Chen, M.L., Shen, F., Huang, W., Qi, J.H., Wang, Y., Feng, Y.Q.*, Liu, S.M.* and Yuan, B.F.* (2013) Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic-Interaction Liquid Chromatography/Quadrupole TOF Mass Spectrometry. Clin Chem, 59, 824-832.

  15. Chen, M.L., Liu, Y.L., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Preparation of a Hyper-cross-linked Polymer Monolithic Column and Its Application to the Sensitive Determination of Genomic DNA Methylation. Chem-Eur J, 19, 1035-1041.

  16. Tang, Y., Gao, X.D., Wang, Y., Yuan, B.F.* and Feng, Y.Q.* (2012) Widespread existence of Cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry. Anal Chem, 84, 7249-7255.

  17. Yuan, B.F., Zhang, J., Wang, H., Xiong, L., Cai, Q., Wang, T., Jacobsen, S., Pradhan, S. and Wang, Y.* (2011) 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1. Cancer Res, 71, 1904-1911.

  18. Yuan, B.F., Wang, J., Cao, H., Sun, R. and Wang, Y.* (2011) High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing. Nucleic Acids Res, 39, 5945-5954.

  19. Yuan, B.F., O''Connor, T.R. and Wang, Y.* (2010) 6-Thioguanine and S(6)-Methylthioguanine Are Mutagenic in Human Cells. ACS Chem. Biol., 5, 1021-1027.

  20. Yuan, B.F., Cao, H., Jiang, Y., Hong, H. and Wang, Y.* (2008) Efficient and accurate bypass of N2-(1-carboxyethyl)-2''-deoxyguanosine by DinB DNA polymerase in vitro and in vivo. Proc. Natl. Acad. Sci. U S A., 105, 8679-8684.

  21. Yuan, B.F., Zhuang, X.Y., Hao, Y.H. and Tan, Z.* (2008) Kinetics of base stacking-aided DNA hybridization. Chem Commun (Camb), 6600-6602.


发表的全部论文可以从下面的连接查看

http://scholar.google.com/citations?hl=enundefinedamp;user=jrzBHhAAAAAJundefinedamp;view_op=list_worksundefinedamp;pagesize=100

http://orcid.org/0000-0001-5223-4659

 

获奖和荣誉

2015  国家自然科学基金优秀青年基金获得者

2011 “楚天学者”特聘教授